異檸檬酸脱氫酶 (NADP+)（英語：isocitrate dehydrogenase (NADP+)，EC 1.1.1.42 （页面存档备份，存于互联网档案馆））是一种以NAD+或NADP+为受体、作用于供体CH-OH基团上的氧化还原酶。这种酶能催化以下酶促反应：
| 異檸檬酸脱氫酶 (NADP+)
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| 大肠杆菌（E. coli）異檸檬酸脱氫酶(NADP+)的晶体结构（PDB 1CW7）[1] 这种酶有三个活性位点，三分子柠檬酸中的其中一个在NADP+结合区域中。
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| 命名
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| 系统命名
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isocitrate:NADP+ oxidoreductase (decarboxylating)
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| 缩写
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IDP; IDP1; IDP2; IDP3; IDH（有歧义）
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| 别名
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oxalosuccinate decarboxylase; isocitrate dehydrogenase (NADP); oxalsuccinic decarboxylase; isocitrate (NADP) dehydrogenase; isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase; NADP-specific isocitrate dehydrogenase; NADP-linked isocitrate dehydrogenase; NADP-dependent isocitrate dehydrogenase; NADP isocitric dehydrogenase; isocitrate dehydrogenase (NADP-dependent); NADP-dependent isocitric dehydrogenase; triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase; NADP+-linked isocitrate dehydrogenase; dual-cofactor-specific isocitrate dehydrogenase; NADP+-ICDH; NADP+-IDH
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| 识别码
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| EC編號
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1.1.1.42
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| CAS号
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9028-48-2
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| 数据库
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| IntEnz
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IntEnz浏览
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| BRENDA
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BRENDA入口
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| ExPASy
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NiceZyme浏览
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| KEGG
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KEGG入口
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| MetaCyc
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代谢路径
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| PRIAM
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概述
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| PDB
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RCSB PDB PDBj PDBe PDBsum
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| 基因本体
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AmiGO / EGO
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- 異檸檬酸 + NADP+
2-酮戊二酸 + CO2 + NADPH
異檸檬酸脱氫酶 (NADP+)需要Mn2+或Mg2+以维持活性。真核细胞中的异柠檬酸脱氢酶 (NAD+)以两种形式存在：其中一种与NADP+还存在于细胞质基质中不能变构调节；另一种与NAD+相关联且仅在线粒体中被发现并但可以变构调节。[2] 这两种酶——异柠檬酸脱氢酶 (NADP+)与异柠檬酸脱氢酶 (NAD+)并不相同，前者能以草酰琥珀酸作为底物。某些物种的异柠檬酸脱氢酶 (NADP+)也能利用NAD+，但催化的反应速率较慢。[3]
参考文献
- ^ Cherbavaz DB, Lee ME, Stroud RM, Koshland DE. Active site water molecules revealed in the 2.1 A resolution structure of a site-directed mutant of isocitrate dehydrogenase. J. Mol. Biol. January 2000, 295 (3): 377–85. PMID 10623532. doi:10.1006/jmbi.1999.3195.
- ^ Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence. 134. 1995: 85–90 [2012-05-08]. PMID 8593959. （原始内容存档于2019-03-23） （英语）.
- ^ Steen, I.H., Lien, T. and Birkeland, N.-K. Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, ‘’Archaeoglobus fulgidus’’.. Arch. Microbiol. 1997, 168: 412–420. PMID 9325430 （英语）.
- Agosin, M.U. and Weinbach, E.C. Partial purification and characterization of the isocitric dehydrogenase from ‘’Trypanosoma cruzi’’.. Biochim. Biophys. Acta. 1956, 21: 117–126. PMID 13363868 （英语）.
- Moyle, J. and Dixon, M. Purification of the isocitrate enzyme (triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase).. Biochem. J. 1956, 63: 548–552. PMID 13355848 （英语）.
- Plaut, G.W.E. Isocitrate dehydrogenases. Lardy, H. and Myrbäck, K. (编). The Enzymes 7 2nd. New York: Academic Press. 1963: 105–126 （英语）.
- Siebert, G., Dubuc, J., Warner, R.C. and Plaut, G.W.E. The preparation of isocitrate dehydrogenase from mammalian heart.. J. Biol. Chem. 1957, 226: 965–975. PMID 13438885 （英语）.
- Vickery, H.B. A. suggested new nomenclature for the isomers of isocitric acid. 237. 1962: 1739–1741. PMID 13925783 （英语）.
- Koh, H.J., Lee, S.M., Son, B.G., Lee, S.H., Ryoo, Z.Y., Chang, K.T., Park, J.W., Park, D.C., Song, B.J., Veech, R.L., Song, H. and Huh, T.L. Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism.. 2004: 39968–39974. PMID 15254034 （英语）.
- Ceccarelli, C., Grodsky, N.B., Ariyaratne, N., Colman, R.F. and Bahnson, B.J. Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism.. J. Biol. Chem. 277. 2002: 43454–43462. PMID 12207025 （英语）.
