異檸檬酸脫氫酶 (NADP+)（英語：isocitrate dehydrogenase (NADP+)，EC 1.1.1.42 （頁面存檔備份，存於網際網路檔案館））是一種以NAD+或NADP+為受體、作用於供體CH-OH基團上的氧化還原酶。這種酶能催化以下酶促反應：
| 異檸檬酸脫氫酶 (NADP+)
|
|
| 大腸桿菌（E. coli）異檸檬酸脫氫酶(NADP+)的晶體結構（PDB 1CW7）[1] 這種酶有三個活性位點，三分子檸檬酸中的其中一個在NADP+結合區域中。
|
| 命名
|
| 系統命名
|
isocitrate:NADP+ oxidoreductase (decarboxylating)
|
| 縮寫
|
IDP; IDP1; IDP2; IDP3; IDH（有歧義）
|
| 別名
|
oxalosuccinate decarboxylase; isocitrate dehydrogenase (NADP); oxalsuccinic decarboxylase; isocitrate (NADP) dehydrogenase; isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase; NADP-specific isocitrate dehydrogenase; NADP-linked isocitrate dehydrogenase; NADP-dependent isocitrate dehydrogenase; NADP isocitric dehydrogenase; isocitrate dehydrogenase (NADP-dependent); NADP-dependent isocitric dehydrogenase; triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase; NADP+-linked isocitrate dehydrogenase; dual-cofactor-specific isocitrate dehydrogenase; NADP+-ICDH; NADP+-IDH
|
|
| 識別碼
|
| EC編號
|
1.1.1.42
|
| CAS號
|
9028-48-2
|
| 資料庫
|
| IntEnz
|
IntEnz瀏覽
|
| BRENDA
|
BRENDA入口
|
| ExPASy
|
NiceZyme瀏覽
|
| KEGG
|
KEGG入口
|
| MetaCyc
|
代謝路徑
|
| PRIAM
|
概述
|
| PDB
|
RCSB PDB PDBj PDBe PDBsum
|
| 基因本體
|
AmiGO / EGO
|
|
|
- 異檸檬酸 + NADP+
2-酮戊二酸 + CO2 + NADPH
異檸檬酸脫氫酶 (NADP+)需要Mn2+或Mg2+以維持活性。真核細胞中的異檸檬酸脫氫酶 (NAD+)以兩種形式存在：其中一種與NADP+還存在於細胞質基質中不能變構調節；另一種與NAD+相關聯且僅在粒線體中被發現並但可以變構調節。[2] 這兩種酶——異檸檬酸脫氫酶 (NADP+)與異檸檬酸脫氫酶 (NAD+)並不相同，前者能以草醯琥珀酸作為底物。某些物種的異檸檬酸脫氫酶 (NADP+)也能利用NAD+，但催化的反應速率較慢。[3]
參考文獻
- ^ Cherbavaz DB, Lee ME, Stroud RM, Koshland DE. Active site water molecules revealed in the 2.1 A resolution structure of a site-directed mutant of isocitrate dehydrogenase. J. Mol. Biol. January 2000, 295 (3): 377–85. PMID 10623532. doi:10.1006/jmbi.1999.3195.
- ^ Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence. 134. 1995: 85–90 [2012-05-08]. PMID 8593959. （原始內容存檔於2019-03-23） （英語）.
- ^ Steen, I.H., Lien, T. and Birkeland, N.-K. Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, ‘’Archaeoglobus fulgidus’’.. Arch. Microbiol. 1997, 168: 412–420. PMID 9325430 （英語）.
- Agosin, M.U. and Weinbach, E.C. Partial purification and characterization of the isocitric dehydrogenase from ‘’Trypanosoma cruzi’’.. Biochim. Biophys. Acta. 1956, 21: 117–126. PMID 13363868 （英語）.
- Moyle, J. and Dixon, M. Purification of the isocitrate enzyme (triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase).. Biochem. J. 1956, 63: 548–552. PMID 13355848 （英語）.
- Plaut, G.W.E. Isocitrate dehydrogenases. Lardy, H. and Myrbäck, K. (編). The Enzymes 7 2nd. New York: Academic Press. 1963: 105–126 （英語）.
- Siebert, G., Dubuc, J., Warner, R.C. and Plaut, G.W.E. The preparation of isocitrate dehydrogenase from mammalian heart.. J. Biol. Chem. 1957, 226: 965–975. PMID 13438885 （英語）.
- Vickery, H.B. A. suggested new nomenclature for the isomers of isocitric acid. 237. 1962: 1739–1741. PMID 13925783 （英語）.
- Koh, H.J., Lee, S.M., Son, B.G., Lee, S.H., Ryoo, Z.Y., Chang, K.T., Park, J.W., Park, D.C., Song, B.J., Veech, R.L., Song, H. and Huh, T.L. Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism.. 2004: 39968–39974. PMID 15254034 （英語）.
- Ceccarelli, C., Grodsky, N.B., Ariyaratne, N., Colman, R.F. and Bahnson, B.J. Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism.. J. Biol. Chem. 277. 2002: 43454–43462. PMID 12207025 （英語）.
