Vero E6

Vero 76細胞衍生的克隆株

Vero E6(亦称Vero C1008)是一种非洲绿猴细胞系,是Vero 76细胞衍生的克隆株,并且在形成单层膜后显示出一定程度的接触抑制英语Contact inhibition作用,故而适合繁殖一些复制缓慢的病毒。当Vero E6细胞被马秋波病毒(Machupo virus)、胡宁病毒英语Argentinian mammarenavirus拉萨病毒英语Lassa mammarenavirus等出血热病毒、马尔堡病毒萨伊埃博拉病毒等病毒感染时,都会表现出细胞病变作用。该细胞系目前已进一步用于麻疹病毒[1]埃博拉病毒[2]克里米亚-刚果出血热病毒[3]严重急性呼吸系统综合症冠状病毒[4]严重急性呼吸系统综合症冠状病毒2型(SARS-CoV-2)[5]等的分离和生长。

细胞系

参考资料

  1. ^ Rota, JS; Hummel, KB; Rota, PA; Bellini, WJ. Genetic variability of the glycoprotein genes of current wild-type measles isolates.. Virology. 1992-05, 188 (1): 135–42 [2020-02-22]. PMID 1566568. doi:10.1016/0042-6822(92)90742-8. (原始内容存档于2020-02-22). 
  2. ^ Sanchez, A; Kiley, MP. Identification and analysis of Ebola virus messenger RNA.. Virology. 1987-04, 157 (2): 414–20 [2020-02-22]. PMID 2881398. doi:10.1016/0042-6822(87)90283-2. (原始内容存档于2020-02-22). 
  3. ^ Saijo, M; Qing, T; Niikura, M; Maeda, A; Ikegami, T; Prehaud, C; Kurane, I; Morikawa, S. Recombinant nucleoprotein-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus.. Journal of clinical microbiology. 2002-05, 40 (5): 1587–91 [2020-02-22]. PMID 11980926. doi:10.1128/jcm.40.5.1587-1591.2002. (原始内容存档于2020-02-22). 
  4. ^ Ng, ML; Tan, SH; See, EE; Ooi, EE; Ling, AE. Proliferative growth of SARS coronavirus in Vero E6 cells.. The Journal of general virology. 2003-12, 84 (Pt 12): 3291–303 [2020-02-22]. PMID 14645910. doi:10.1099/vir.0.19505-0. (原始内容存档于2020-02-22). 
  5. ^ Wang, Manli; Cao, Ruiyuan; Zhang, Leike; Yang, Xinglou; Liu, Jia; Xu, Mingyue; Shi, Zhengli; Hu, Zhihong; Zhong, Wu; Xiao, Gengfu. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Research. 2020-02-04 [2020-02-22]. doi:10.1038/s41422-020-0282-0. (原始内容存档于2020-02-22).