體細胞核移植

體細胞核移植（Somatic cell nuclear transfer，簡稱：SCNT），又稱體細胞克隆，是遺傳學及發育生物學實驗之中一種使用體細胞和卵細胞培育胚胎的常用技術手段。該技術提取去核卵母細胞，並把供體體細胞核移入卵母細胞中。體細胞核移植技術被應用於臨床治療及克隆技術中。世界上第一隻克隆哺乳動物多利羊即是依靠體細胞核移植技術誕生。2018年1月，中國科學院神經科學研究所非人靈長類平台的研究團隊宣佈成功通過體細胞核移植技術克隆出兩隻食蟹猴中中、華華。^[1]

介紹

細胞核移植技術，就是將供體細胞核移入除去核的卵母細胞中，使後者不經過精子穿透等有性過程即無性繁殖即可被激活、分裂並發育成新個體，使得核供體的基因得到完全複製。^[2]1996年世界上第一個使用該技術克隆的動物多利羊誕生。^[3]

參考文獻

^ Liu, Zhen; et al. Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer. Cell (journal). 24 January 2018 [24 January 2018]. doi:10.1016/j.cell.2018.01.020. （原始內容存檔於2018-02-08）.
^ Somatic cell nuclear transfer | biology and technology. Encyclopedia Britannica. [27 January 2018]. （原始內容存檔於2018-01-28）（英語）.
^ Somatic Cell Nuclear Transfer - Embryology. embryology.med.unsw.edu.au. [27 January 2018]. （原始內容存檔於2018-01-28）.

延伸閱讀

Wilmut I, Beaujean N, de Sousa PA, et al. Somatic cell nuclear transfer. Nature. October 2002, 419 (6907): 583–6. PMID 12374931. doi:10.1038/nature01079.
Kikyo N, Wolffe AP. Reprogramming nuclei: insights from cloning, nuclear transfer and heterokaryons. J. Cell Sci. 113. January 2000,. ( Pt 1): 11–20. PMID 10591621.
Tian XC, Kubota C, Enright B, Yang X. Cloning animals by somatic cell nuclear transfer—biological factors. Reprod. Biol. Endocrinol. November 2003, 1 (1): 98. PMC 521203  . PMID 14614770. doi:10.1186/1477-7827-1-98.
Gurdon JB, Byrne JA, Simonsson S. Nuclear reprogramming and stem cell creation. Proc. Natl. Acad. Sci. U.S.A. 100. September 2003,. Suppl 1 (90001): 11819–22. PMC 304092  . PMID 12920185. doi:10.1073/pnas.1834207100.

參見

外部連結

Research Cloning: Medical and scientific, legal and ethical aspects^{[失效連結]}
The Basics: Stem Cells and Public Policy The Century Foundation, June 2005
"Research Cloning Basic Science", Center for Genetics and Society, (Last modified October 4, 2004, retrieved October 6, 2006)
Cloning: present uses and promises National Institutes of Health, Paper giving background information on cloning in general and SCNT from The Office of Science Policy Analysis.
Nuclear Transfer – Stem Cells or Somatic Cell Nuclear Transfer (SCNT) The International Society for Stem Cell Research
The Hinxton Group: An International Consortium on Stem Cells, Ethics & Law（頁面存檔備份，存於互聯網檔案館）

[CELL-20180124-1] Liu, Zhen; et al. Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer. Cell (journal). 24 January 2018 [24 January 2018]. doi:10.1016/j.cell.2018.01.020. （原始內容存檔於2018-02-08）.

[2] Somatic cell nuclear transfer | biology and technology. Encyclopedia Britannica. [27 January 2018]. （原始內容存檔於2018-01-28）（英語）.

[3] Somatic Cell Nuclear Transfer - Embryology. embryology.med.unsw.edu.au. [27 January 2018]. （原始內容存檔於2018-01-28）.

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