四环素转录调控系统

四环素转录调控系统（Tetracycline-Controlled Transcriptional Activation）是一种能够定时调控（开启或关闭）特定基因表达的转录调控系统。该系统由一个顺式作用元件和一个活性受四环素调控的特殊转录调控蛋白（反式作用因子）构成。当四环素或多西环素（Doxy）等四环素衍生物存在时，处于该系统调控下的基因表达会开启或关闭^[1]^[2]。四环素转录调控系统基于革兰氏阴性菌四环素抗性基因表达调控系统。四环素转录调控系统中，转录调控蛋白即是通过原核生物的TetA或TetR蛋白与病毒的转录激活蛋白结构域融合产生，而与之配对的顺式作用原件则是以细菌的四环素抗性基因操纵子为基础构建的。四环素转录调控系统分为两种类型：Tet-On系统和Tet-Off系统。在Tet-On系统中，有四环素类化合物存在时，对应的基因表达会打开。而在Tet-Off系统中，有四环素类化合物存在时，对应基因的表达会关闭^[3]。

参见

参考

^ Dong X. Yin, Robert T. Schimke; et al. Tetracycline-Controlled Gene Expression System Achieves High-Level and Quantitative Control of Gene Expression. Analytical Biochemistry 235, 195-201 (1996), Ariticle No.0112.
^ Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H. Transcriptional activation by tetracyclines in mammalian cells. Science. 1995, 268 (5218): 1766–1769. PMID 7792603. doi:10.1126/science.7792603.
^ Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system (PDF). Nature Structural & Molecular Biology. 2000, 7 (3): 215–219 [2017-04-04]. PMID 10700280. doi:10.1038/73324. （原始内容存档 (PDF)于2016-10-06）.

[1] Dong X. Yin, Robert T. Schimke; et al. Tetracycline-Controlled Gene Expression System Achieves High-Level and Quantitative Control of Gene Expression. Analytical Biochemistry 235, 195-201 (1996), Ariticle No.0112.

[pmid7792603-2] Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H. Transcriptional activation by tetracyclines in mammalian cells. Science. 1995, 268 (5218): 1766–1769. PMID 7792603. doi:10.1126/science.7792603.

[pmid10700280-3] Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system (PDF). Nature Structural & Molecular Biology. 2000, 7 (3): 215–219 [2017-04-04]. PMID 10700280. doi:10.1038/73324. （原始内容存档 (PDF)于2016-10-06）.

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