AvaI是一种发现于细菌多变鱼腥藻Anabaena variabilis)的限制酶,与发现于地芽胞杆菌属细菌(Geobacillus stearothermophilus)的BsoBI同裂酶[1],它们的识别序列为:

Endonuc-BsobI
限制酶BsoBI与DNA复合体的结构
鉴定
标志Endonuc-BsobI
PfamPF09194旧版
InterPro英语InterProIPR015277
SCOP英语Structural Classification of Proteins1dc1 / SUPFAM
5'CYCGRG
3'GRGCYC

其中Y为任意嘧啶,R为任意嘌呤,切割产生的粘性末端为:

5'---C     YCGRG---3'
3'---GRGCY     C---5'

参考文献

  1. ^ van der Woerd MJ, Pelletier JJ, Xu S, Friedman AM. Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis. Structure. February 2001, 9 (2): 133–44. PMID 11250198. doi:10.1016/S0969-2126(01)00564-0 .