人脐静脉内皮细胞

人脐静脉内皮细胞（Human umbilical vein endothelial cell、HUVEC）是源自脐带静脉内皮的细胞系，最早在1970年代进行分离，并且在体外培养^[1] 。它们可以用作研究内皮细胞功能和病理学（例如血管新生）的实验模型^[2] 。使用它们的原因包括成本低，并且简单的技术就可将它们与通常在分娩后切除的脐带分离。 HUVEC细胞很容易就可以在实验室内增殖。它们像人脐动脉内皮细胞（HUAEC）一样，当衬砌血管壁时就会表现出鹅卵石状表型。

HUVEC细胞的角蛋白细胞骨架的显微图像

目前已知Anip973培养上清液对HUVEC细胞具有促分裂增殖作用^[3]^[4]，机制可以是培养上清液内含的细胞因子激活了周期蛋白D激酶、导致周期蛋白D（英语：Cyclin D）和周期蛋白依赖性激酶的高度表达，从而诱导更多细胞进入S期，同时增加去氧核糖核酸的合成^[4]。

参考资料

^ Jiménez, N; Krouwer, VJ; Post, JA. A new, rapid and reproducible method to obtain high quality endothelium in vitro.. Cytotechnology. 2013-01, 65 (1): 1–14 [2020-02-20]. PMID 22573289. doi:10.1007/s10616-012-9459-9. （原始内容存档于2020-02-20）.
^ Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB. Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis. Stem Cell Rev. 2006, 2 (2): 93–102. PMID 17237547. doi:10.1007/s12015-006-0015-x.
^ Wang Ji; Zhang Qian; Xie Hua; Gu LiGang; Niu XuYan; Liu LinTao. Effect of Hydroxy Safflor Yellow A on the proliferation of human umbilical vein endothelial cells with the stimulus of tumor cell. China Journal of Traditional Chinese Medicine and Pharmacy. 2009, 24 (5): 572-575 [2020-02-20]. （原始内容存档于2020-02-20）.
^ ^4.0 ^4.1 Zhang, C; Li, Y; Wang, J; Li, K. [The Supernatant Obtained from Cultured Anip973 Cells Enhances the Biological Activities of HUVEC].. Zhongguo fei ai za zhi = Chinese journal of lung cancer. 2015-11, 18 (11): 668–73 [2020-02-20]. PMID 26582221. doi:10.3779/j.issn.1009-3419.2015.11.02.

外部链接

医学主题词表（MeSH）：Human umbilical vein endothelial cells
Understanding the Vasculature with the Help of HUVECs （页面存档备份，存于互联网档案馆）
Primary Umbilical Vein Endothelial Cells (Normal, Human) （页面存档备份，存于互联网档案馆） at ATCC

[1] Jiménez, N; Krouwer, VJ; Post, JA. A new, rapid and reproducible method to obtain high quality endothelium in vitro.. Cytotechnology. 2013-01, 65 (1): 1–14 [2020-02-20]. PMID 22573289. doi:10.1007/s10616-012-9459-9. （原始内容存档于2020-02-20）.

[pmid17237547-2] Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB. Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis. Stem Cell Rev. 2006, 2 (2): 93–102. PMID 17237547. doi:10.1007/s12015-006-0015-x.

[3] Wang Ji; Zhang Qian; Xie Hua; Gu LiGang; Niu XuYan; Liu LinTao. Effect of Hydroxy Safflor Yellow A on the proliferation of human umbilical vein endothelial cells with the stimulus of tumor cell. China Journal of Traditional Chinese Medicine and Pharmacy. 2009, 24 (5): 572-575 [2020-02-20]. （原始内容存档于2020-02-20）.

[Zhang-4] 4.0 ^4.1 Zhang, C; Li, Y; Wang, J; Li, K. [The Supernatant Obtained from Cultured Anip973 Cells Enhances the Biological Activities of HUVEC].. Zhongguo fei ai za zhi = Chinese journal of lung cancer. 2015-11, 18 (11): 668–73 [2020-02-20]. PMID 26582221. doi:10.3779/j.issn.1009-3419.2015.11.02.

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