Calu-3细胞

Calu-3是经常应用于癌症研究和药物开发的人类肺癌细胞系，最初于1975年由纪念斯隆-凯特琳癌症中心科研人员分离自一名25岁高加索男性肺癌患者的胸腔积液。

特征

ERBB2基因在Calu-3细胞中过度表达，并在侵袭性癌症的扩散中发挥作用。

Calu-3细胞以粘附的单层形式生长及增殖，而且对CK-7（英语：Keratin 7）、封闭蛋白（英语：Occludin）和E-钙粘蛋白呈阳性反应，同时具有大量的囊肿性纤维化跨膜调节器（英语：Cystic fibrosis transmembrane conductance regulator）^[1]，并且因过度表达ERBB2基因而令ErbB2/Her2被激活^[2]。

科研用途

Calu-3细胞可以在临床前研究中充当呼吸系统模型，并且因其对异物的反应能力强而成为吸气及肺损伤模型^[3]。多份研究指出Calu-3细胞通常用作体外和体内抗肺癌药物开发的模型^[3]、显示摄入低分子量物质的能力而有助于肺部药物的输送的研究^[4]、研究肺上皮细胞分泌氯离子的过程^[1]。Calu-3细胞可以应用于重点在肺细胞屏障完整性和表面蛋白表达的高速化合物筛选（英语：High-throughput screening）^[5]。

参考资料

^ ^1.0 ^1.1 Shen, BQ; Finkbeiner, WE; Wine, JJ; Mrsny, RJ; Widdicombe, JH. Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl- secretion.. The American journal of physiology. 1994-05, 266 (5 Pt 1): L493–501 [2019-12-10]. PMID 7515578. doi:10.1152/ajplung.1994.266.5.L493.
^ Kreft, ME; Jerman, UD; Lasič, E; Hevir-Kene, N; Rižner, TL; Peternel, L; Kristan, K. The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2015-03-10, 69: 1–9 [2019-12-10]. PMID 25555374. doi:10.1016/j.ejps.2014.12.017.
^ ^3.0 ^3.1 Zhu, Y; Chidekel, A; Shaffer, TH. Cultured human airway epithelial cells (calu-3): a model of human respiratory function, structure, and inflammatory responses.. Critical care research and practice. 2010, 2010 [2019-12-10]. PMID 20948883. doi:10.1155/2010/394578.
^ Foster, KA; Avery, ML; Yazdanian, M; Audus, KL. Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery.. International journal of pharmaceutics. 2000-11-04, 208 (1-2): 1–11 [2019-12-10]. PMID 11064206. doi:10.1016/s0378-5173(00)00452-x.
^ Haghi, M; Young, PM; Traini, D; Jaiswal, R; Gong, J; Bebawy, M. Time- and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model.. Drug development and industrial pharmacy. 2010-10, 36 (10): 1207–14 [2019-12-10]. PMID 20374185. doi:10.3109/03639041003695113.

外部链接

Cellosaurus entry for Calu-3（页面存档备份，存于互联网档案馆）

[b-1] 1.0 ^1.1 Shen, BQ; Finkbeiner, WE; Wine, JJ; Mrsny, RJ; Widdicombe, JH. Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl- secretion.. The American journal of physiology. 1994-05, 266 (5 Pt 1): L493–501 [2019-12-10]. PMID 7515578. doi:10.1152/ajplung.1994.266.5.L493.

[2] Kreft, ME; Jerman, UD; Lasič, E; Hevir-Kene, N; Rižner, TL; Peternel, L; Kristan, K. The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2015-03-10, 69: 1–9 [2019-12-10]. PMID 25555374. doi:10.1016/j.ejps.2014.12.017.

[a-3] 3.0 ^3.1 Zhu, Y; Chidekel, A; Shaffer, TH. Cultured human airway epithelial cells (calu-3): a model of human respiratory function, structure, and inflammatory responses.. Critical care research and practice. 2010, 2010 [2019-12-10]. PMID 20948883. doi:10.1155/2010/394578.

[4] Foster, KA; Avery, ML; Yazdanian, M; Audus, KL. Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery.. International journal of pharmaceutics. 2000-11-04, 208 (1-2): 1–11 [2019-12-10]. PMID 11064206. doi:10.1016/s0378-5173(00)00452-x.

[5] Haghi, M; Young, PM; Traini, D; Jaiswal, R; Gong, J; Bebawy, M. Time- and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model.. Drug development and industrial pharmacy. 2010-10, 36 (10): 1207–14 [2019-12-10]. PMID 20374185. doi:10.3109/03639041003695113.

[1]

[2]

[3]

[4]

[5]