Calu-3細胞

Calu-3是經常應用於癌症研究和藥物開發的人類肺癌細胞系，最初於1975年由紀念斯隆-凱特琳癌症中心科研人員分離自一名25歲高加索男性肺癌患者的胸腔積液。

特徵

ERBB2基因在Calu-3細胞中過度表達，並在侵襲性癌症的擴散中發揮作用。

Calu-3細胞以粘附的單層形式生長及增殖，而且對CK-7（英語：Keratin 7）、封閉蛋白（英語：Occludin）和E-鈣粘蛋白呈陽性反應，同時具有大量的囊腫性纖維化跨膜調節器（英語：Cystic fibrosis transmembrane conductance regulator）^[1]，並且因過度表達ERBB2基因而令ErbB2/Her2被激活^[2]。

科研用途

Calu-3細胞可以在臨床前研究中充當呼吸系統模型，並且因其對異物的反應能力強而成為吸氣及肺損傷模型^[3]。多份研究指出Calu-3細胞通常用作體外和體內抗肺癌藥物開發的模型^[3]、顯示攝入低分子量物質的能力而有助於肺部藥物的輸送的研究^[4]、研究肺上皮細胞分泌氯離子的過程^[1]。Calu-3細胞可以應用於重點在肺細胞屏障完整性和表面蛋白表達的高速化合物篩選（英語：High-throughput screening）^[5]。

參考資料

^ ^1.0 ^1.1 Shen, BQ; Finkbeiner, WE; Wine, JJ; Mrsny, RJ; Widdicombe, JH. Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl- secretion.. The American journal of physiology. 1994-05, 266 (5 Pt 1): L493–501 [2019-12-10]. PMID 7515578. doi:10.1152/ajplung.1994.266.5.L493.
^ Kreft, ME; Jerman, UD; Lasič, E; Hevir-Kene, N; Rižner, TL; Peternel, L; Kristan, K. The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2015-03-10, 69: 1–9 [2019-12-10]. PMID 25555374. doi:10.1016/j.ejps.2014.12.017.
^ ^3.0 ^3.1 Zhu, Y; Chidekel, A; Shaffer, TH. Cultured human airway epithelial cells (calu-3): a model of human respiratory function, structure, and inflammatory responses.. Critical care research and practice. 2010, 2010 [2019-12-10]. PMID 20948883. doi:10.1155/2010/394578.
^ Foster, KA; Avery, ML; Yazdanian, M; Audus, KL. Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery.. International journal of pharmaceutics. 2000-11-04, 208 (1-2): 1–11 [2019-12-10]. PMID 11064206. doi:10.1016/s0378-5173(00)00452-x.
^ Haghi, M; Young, PM; Traini, D; Jaiswal, R; Gong, J; Bebawy, M. Time- and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model.. Drug development and industrial pharmacy. 2010-10, 36 (10): 1207–14 [2019-12-10]. PMID 20374185. doi:10.3109/03639041003695113.

外部連結

Cellosaurus entry for Calu-3（頁面存檔備份，存於網際網路檔案館）

[b-1] 1.0 ^1.1 Shen, BQ; Finkbeiner, WE; Wine, JJ; Mrsny, RJ; Widdicombe, JH. Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl- secretion.. The American journal of physiology. 1994-05, 266 (5 Pt 1): L493–501 [2019-12-10]. PMID 7515578. doi:10.1152/ajplung.1994.266.5.L493.

[2] Kreft, ME; Jerman, UD; Lasič, E; Hevir-Kene, N; Rižner, TL; Peternel, L; Kristan, K. The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function.. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2015-03-10, 69: 1–9 [2019-12-10]. PMID 25555374. doi:10.1016/j.ejps.2014.12.017.

[a-3] 3.0 ^3.1 Zhu, Y; Chidekel, A; Shaffer, TH. Cultured human airway epithelial cells (calu-3): a model of human respiratory function, structure, and inflammatory responses.. Critical care research and practice. 2010, 2010 [2019-12-10]. PMID 20948883. doi:10.1155/2010/394578.

[4] Foster, KA; Avery, ML; Yazdanian, M; Audus, KL. Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery.. International journal of pharmaceutics. 2000-11-04, 208 (1-2): 1–11 [2019-12-10]. PMID 11064206. doi:10.1016/s0378-5173(00)00452-x.

[5] Haghi, M; Young, PM; Traini, D; Jaiswal, R; Gong, J; Bebawy, M. Time- and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model.. Drug development and industrial pharmacy. 2010-10, 36 (10): 1207–14 [2019-12-10]. PMID 20374185. doi:10.3109/03639041003695113.

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